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Förslaget inkom 2010-04-07

Perfusion in Chinese Hamster Ovary cell cultivation producing monoclonal antibody for biopharmaceutical bioprocess application

OBS! ANSÖKNINGSTIDEN FÖR DETTA EXJOBB HAR LÖPT UT.
The cultivation of Chinese Hamster Ovary cells, CHO, is widely used in the biopharmaceutical industry for the production of drug proteins. Perfusion is a mode of operation where a continuous replacement of the conditioned medium by fresh medium is operated. It has the advantage of allowing high cell densities and therefore increased higher product titres. This mode of operations is also required for some instable proteins since it allows the immediate storage of the cell-free supernatant containing the product of interest at low temperature where the proteolysis is not active.
Our group has developed a bioreactor system perfused with an Alternating Tangential Flow filtration system and shown cell densities as high as 48 * 1E6 cells/mL using a CHO cell line. The purpose of the present work is to develop a perfusion system using disposable bioreactor and to compare different perfusion technologies. Monoclonal antibody producing CHO cell lines will be used for the investigation. The project will be performed in close collaboration with and sponsored by a well-established company. The practical work will be performed at the Royal Institute of Technology in Stockholm.


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