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Does the cannabinoid receptor ligand hemopressin induce cell death in lymphoma?
Mantle cell lymphoma is a type of Non-Hodgkin B cell lymphoma comprising approximately 8% of malignant lymphomas. It has one of the worst prognoses of lymphomas with a median survival of 3-5 years and therefore new therapies are needed. The initiating event in the molecular pathogenesis of MCL is a translocation (11;14), which results in the overexpression of protein called cyclin D1, which participates in regulation of cell cycle. However, other genetic aberrations than t(11;14) also occur. We have previously found that cannabinoid receptors CB1 and CB2 are highly expressed in MCL and that activating them causes cell death of malignant but not normal B cells.
Most ligands to CB receptors are lipids or synthetic potent agonists. Hemopressin is so far the only identified peptide with affinity to CB1. Hemopressin is a 9 amino acid peptide derived from alpha-hemoglobin, the main constituent of red blood cells.
This study aims to evaluate the effect of hemopressin on the viability of MCL and normal B cells. The student will also investigate cannabinoid-induced pathways leading to growth arrest or cell death of lymphoma cells.
Materials and Methods:
MCL cell lines and cell lines derived from normal B lymphocytes
Tumor cells and normal B lymphocytes isolated from patients
Cell viability assay based on spectrophotometric readout (XTT)
Western blotting (including sample preparation and electrophoresis)
This project will provide important new knowledge on the role of cannabinoid receptors and cannabinoids in the pathogenesis of MCL and might have clinical implications.
Informationen om uppsatsförslag är hämtad från Nationella Exjobb-poolen.