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Förslaget inkom 2011-06-16

Structural studies of a bacterial lipopolysaccharide

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Background
Lipopolysaccharides are the major constituents of the cell walls of Gram-negative bacteria. The lipopolysaccharide has a long polysaccharide “tail”, which is anchored to the cell wall via a core oligosaccharide and fatty acids attached to sugar units. The polysaccharide part (also called an antigen) is detected by the immune system, which produces antibodies against that specific antigen. Knowledge about the structure of lipopolysaccharides can be used to produce new synthetic vaccines.

The project
The project includes chemical degradation, purification and structural investigation of a lipopolysaccharide. The techniques used will be gel filtration for purification and nuclear magnetic resonance (NMR), gas chromatography-mass spectrometry (GC-MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) for structural elucidation.

About us
We are a small, but well-equipped department at the Swedish University of Agricultural Sciences in Uppsala. The department belongs to the Biocenter cluster and will move to a new-built building with new instruments in September 2011.

Requested competence
Knowledge equivalent to 30 hp at advanced level (A1N, A1F) in Chemistry. Approved Bachelor thesis/Independent project in the first cycle or a Degree of Bachelor.
Knowledge equivalent to English B from upper secondary school.

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