Förslaget inkom 2007-04-27
Cardiac disease and heart valve amyloidosis-what is the connection?
OBS! ANSÖKNINGSTIDEN FÖR DETTA EXJOBB HAR LÖPT UT.
The formation of amyloid and other protein deposits in vivo is synonymous with many pathological conditions such as Alzheimer's disease, Creutzfeldt-Jakob disease, ”Skellefteå sjukan” and Parkinson's disease. Amyloid deposits are formed when normally soluble proteins start to aggregate into unsoluble protein fibrils. Different proteins have been demonstrated to have similar fibrils, indicating that fibril formation is accompanied by structural conversion. The mechanism of amyloidogenesis is not clear. In some cases the fibril formation might depend on overproduction or increased concentration of wild type amyloid fibril protein precursor. Another mechanism might be post-translational modifications of amyloid fibrils proteins via proteolysis, conformational changes and / or glycosylation. Heart valve amyloid is found at the high incidence of 53 % in surgically removed heart valves and is often seen in association with cardiac diseases. It is a significant correlation between large amounts of amyloid, hyalin and calcium phosphate. Most amyloid is found in stenotic valves. The aim of this project is to investigate the prevalence of this type of amyloid in heart valves, its nature and its relevance in cardiac disease.
In this project we try to identify the amyloidogenic protein in heart valves by diverse protein techniques like gel chromatography, HPLC, gel electrophoresis, Western blot, mass spectrometry and Edman degradation, electron microscopy and immunohistochemistry. This is a new project with a lot of experiments that have to be performed and therefore we are grateful for all the help we can get from interested students!