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Förslaget inkom 2008-05-22

Peptide-protein interactions

OBS! ANSÖKNINGSTIDEN FÖR DETTA EXJOBB HAR LÖPT UT.
In proinsulin, C-peptide constitutes a connecting segment between the B and A chains of insulin. After processing, it is released with insulin from the pancreatic beta cells. C-peptide is recognized to be a bioactive peptide as evidenced by
o cellular and signalling effects,
o effects in animal experiments,
o in clinical studies of patients with type 1 diabetes, and most recently,
o in disaggregation of insulin oligomers.

C-peptide binds specifically to cell membranes from pancreatic beta cells, human renal tubular cells, human fibroblasts and endothelial cells. The observations indicate that these cells may have membrane receptors for C-peptide, often suggested to be G-protein coupled receptors (GPCRs) as deduced from effects of pertussis toxin (PTX). We have furthermore showed that C-peptide is internalized in a specific manner.

We are studying the interactions of C-peptide with other proteins using
o subcellular fractionation
o affinity-based methods (magnetic beads, immunoprecipitation)
o Biacore
o mass spectrometry
o crosslinking
o other relevant methods


Are you interested in participating, please contact: Emma.Lindahl@ki.se, 08-524 87 688

Department for Medical Biochemistry and Biophysics, KI Solna
Supervisors: professor Hans Jörnvall and PhD-student Emma Lindahl


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