PHAGE AS THERAPEUTIC AGENTS
We have isolated human E. coli strains from patients with urinary tract infections and diarrhea, and tested these with different T4-like phage. Results sofar indicate profound differences in phage development depending primarily on the host infected. The goal of the present project is to determine what causes these differences, and what makes phage successful predators of different hosts in infection under conditions mimicking those in the human gut. This will be done by analyzing mRNA differential display, and isolating and characterizing mutants able to kill bacteria more or less efficiently than wild-type phage under the tested conditions.
Methods employed include anaerobic cultivation, biological infectivity assays, mutagenesis and mutant analyses, RNA isolation, Northern blots, PhosphorImager quantification.
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