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Förslaget inkom 2009-06-01

Study of the perfusion by ATF filtration system for perfusion and total medium change of a Chinese Hamster Ovary cell cultivation

OBS! ANSÖKNINGSTIDEN FÖR DETTA EXJOBB HAR LÖPT UT.
The cultivation of Chinese Hamster Ovary cells, CHO, is widely used in the biopharmaceutical industry for the production of drug proteins. Perfusion is a mode of operation where a continuous replacement of the conditioned medium by fresh medium is operated. It has the advantage of allowing high cell densities and therefore increased higher product titres. This mode of operations is also required for some instable proteins since it allows the immediate storage of the cell-free supernatant containing the product of interest at low temperature where the proteolysis is not active. The ATF filtration device has been designed to perfuse mammalian cell cultivation process and has been recently commercially available.
The purpose of the present work is to study perfusion and total medium change using the ATF filtration unit in bioreactor with CHO cell line. This process will be representative of industrial processes used for the production of biopharmaceuticals. The work will mainly focus on studying the parameters of the ATF system for optimal perfusion and total medium change operations, identification of the limitations, etc. Finally if time allows, the system will be applied on another bioreactor system, the disposable Wave Bioreactor. The work will be performed in collaboration with GE Healthcare (Sweden) and the cultivation medium will sponsored by Irvine Scientific (CA, USA).


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