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Chlamydiae - how to trace who has been with whom. An unsexy task about sexual infections
We have recently performed a major study where DNA sequencing of the ompA gene in C. trachomatis was used to improve the contact tracing work and the epidemiological understanding of sexual networks (Lysén et al, J Clin Microbiol 2004, 42:1641-7). Although 29 different genotypes of C. trachomatis were identified ompA sequencing does not provide enough variation for strain separation. In order to increase the resolution of molecular epidemiology new methods are needed. We have initiated the development of a multi locus sequence typing system and have achieved very promising results. Now we explore the chlamydia genome for further target regions.
Task: First, to develop a sequence based multi locus target system for identification of C. trachomatis strains.
The work consists of PCR and DNA sequencing technology, where candidate loci for sequencing will be tested. Bioinformatics is used for identification of the optimal sequence targets and to achieve the highest resolution of the genotyping system.
Second, to design a method for application of the typing system and eveluated it on a clinical contact tracing material.
Third, explore obtained sequence information for phylogenetic analysis of chlamydiae.
If expected results are achieved you will be coauthor of a publication.
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